Microtubule heterogeneity of Ornithogalum umbellatum ovary epidermal cells: non-stable cortical microtubules and stable lipotubuloid microtubules

Microtubule heterogeneity of Ornithogalum umbellatum ovary epidermal cells: non-stable cortical microtubules and stable lipotubuloid microtubules

Blog Article

Lipotubuloids, structures containing lipid bodies and microtubules, are described in ovary epidermalcells of Ornithogalum umbellatum.Microtubules of lipotubuloids can be fixed in electron microscope fixativecontaining only buffered OsO4 or in glutaraldehyde with OsO4 post-fixation, or in a mixture of OsO4 and glutaraldehyde[1].None of these substances fixes cortical microtubules of ovary epidermis of this plant which ischaracterized by dynamic longitudinal growth.

However, cortical microtubules can be sgt grit discount promotional code fixed with cold methanolaccording immunocytological methods with the use of b-tubulin antibodies and fluorescein.The existence ofcortical microtubules has also been evidenced by EM observations hemi procharger solely after the use of taxol, microtubulestabilizer, and fixation in a glutaraldehyde/OsO4 mixture.These microtubules mostly lie transversely, sometimesobliquely, and rarely parallel to the cell axis.

Staining, using Ruthenium Red and silver hexamine, has revealedthat lipotubuloid microtubules surface is covered with polysaccharides.The presumption has been made thatthe presence of a polysaccharide layer enhances the stability of lipotubuloid microtubules.